Transient expression of genes introduced into cultured cells of Drosophila.
نویسندگان
چکیده
منابع مشابه
Transient expression of Drosophila melanogaster rDNA promoter into cultured Drosophila cells.
Recombinant plasmids that carry the bacterial CAT gene under the transcriptional control of the D. melanogaster rDNA promoter have been introduced by transfection into cultured Schneider II Drosophila cells and their template activity followed at the RNA and protein level. While no CAT enzyme activity is measurable 48 hrs after transfection, high levels of hybrid rRNA-CAT transcripts that origi...
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15 صفحه اولTransient expression of DNA after ballistic introduction into Drosophila embryos.
Current procedures for introduction of DNA into Drosophila embryos require the time-consuming microinjection of DNA solution into individual embryos (1, 2). A procedure has recently been used successfully in plants, yeast and cultured cells for the ballistic introduction of DNA via tungsten (W) particles (3-5) . Using a modification of this procedure for Drosophila embryos, we have obtained tra...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.23.7095